: The latest edition includes taxonomic updates and new tables for organisms likely identified through advanced technologies like MALDI-TOF mass spectrometry.
Online forums and document-sharing sites often host files labeled "M45.pdf." These are frequently dangerous. A 2023 survey of clinical labs found that 18% admitted using an outdated or unauthorized CLSI document. clsi document m45 pdf
: Abiotrophia and Granulicatella species (nutritionally variant streptococci), Aeromonas species, Pasteurella species, and the HACEK group (e.g., Aggregatibacter , Cardiobacterium ). : The latest edition includes taxonomic updates and
| Edition | Year | Key Changes | |---------|------|--------------| | M45-A | 2005 | First edition; introduced methods for 22 organism groups. | | M45-A2 | 2010 | Added breakpoints for Burkholderia cepacia , Stenotrophomonas maltophilia . | | M45-A3 | 2015 | Expanded to include Aeromonas , Vibrio , and Plesiomonas . Updated QC strains. | | | 2020+ | Current edition. Includes new breakpoints for Campylobacter , revised Haemophilus testing, and quality control updates. | | | M45-A3 | 2015 | Expanded to
The document provides recommendations for the testing of antimicrobial agents against bacteria that are infrequently isolated or require special handling. It covers testing methods, interpretive criteria, and quality control (QC) procedures.
Unlike M100, M45 does not always provide “S/I/R” (Susceptible/Intermediate/Resistant) breakpoints for every drug-organism pair due to limited clinical data. Instead, you may find: